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Lipid Profiling of Algae Biofuel Feedstock Grown in Wastewater

Title: Lipid Profiling of Algae Biofuel Feedstock Grown in Wastewater.
Name(s): Baxter, Alexis, author
Chemistry and Biochemistry
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2012
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Algae represent a carbon neutral feedstock for biofuel production. However, to fully realize the benefits of coupling algae production with nutrient reduction of wastewater, maximum usable biofuel output must be achieved. Indeed, identification of appropriate algal strains, understanding biochemical pathways, and optimization of production of fuel precursors (i.e. lipids) have been identified as some of the most important challenges that need to be addressed before this technology becomes economically feasible. Prospective biofuels originate from the medium-chain fatty acids encased in algal cell membranes. Under ideal conditions, algae synthesize primarily proteins and carbohydrates that are necessary for cell growth. However, when stressed, algae will synthesize large quantities of triacylglycerols (TAGs) that are comprised of mid- to long-chain fatty acids (FAs) bound to glycerol, and these FAs are the primary source of biodiesel. By manipulating growth conditions (e.g. temperature, light, nutrients, pH etc.) the concentration and composition of the FAs can be altered. In this work, various species of freshwater algae grown on domestic wastewater were harvested to determine their potential as a source of combustible biofuels. The goal was to determine which algal strains and stress conditions optimize lipid composition. Total lipid content in dried algal pellets was screened with UV-Vis spectroscopy after derivatization of free fatty acids. Since the quality of the lipids produced is also important in determining biofuel conversion efficiency, the composition of individual lipids (e.g. carbon chain length, number and position of double bonds) was characterized by capillary gas chromatography.
Identifier: FSU_migr_uhm-0102 (IID)
Submitted Note: A Thesis submitted to the Department of Chemistry in partial fulfillment of the requirements for graduation with Honors in the Major.
Degree Awarded: Spring Semester, 2012.
Date of Defense: April 13, 2012.
Subject(s): Biochemistry
Persistent Link to This Record:
Owner Institution: FSU
Is Part of Series: Honors Theses.

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Baxter, A. (2012). Lipid Profiling of Algae Biofuel Feedstock Grown in Wastewater. Retrieved from