You are here

Sensitive, Specific, and Robust Enzyme-linked Immunosorbent Assy (elisa) for Macadamia Nut Seed Protein Detection

Title: A Sensitive, Specific, and Robust Enzyme-linked Immunosorbent Assy (elisa) for Macadamia Nut Seed Protein Detection.
61 views
27 downloads
Name(s): Katz, Julia, author
Nutrition, Food, & Exercise Science
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2011
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: This research project focused on macadamia nut detection. Two specific aims were proposed: (i) develop a competitive inhibition ELISA with a sensitivity of 10 ppm (ii) assess the assay specificity and robustness. The assay specificity of the rabbit anti-macadamia pAb-based ELISA was evaluated by determining assay interference by several select food matrices. The assay robustness was assessed by determining its ability to detect the presence of proteins in variously processed nut seeds. The optimized ELISA has a detection range of 3.2 to 400 ng of macadamia nut protein/mL of BSB. The protein G-purified rabbit anti-macadamia nut protein polyclonal antibodies did not exhibit detectable cross-reactivity with tested protein extracts prepared from several seed and food matrices. The assay was able to detect the seed proteins extracted from macadamia nut seeds subjected to select thermal processing methods.
Identifier: FSU_migr_uhm-0020 (IID)
Keywords: ELISA, Macademia nut seed, Protein, Immunoreactivity
Submitted Note: A Thesis submitted to the Department of Nutrition, Food and Exercise Sciences in partial fulfillment of the requirements for graduation with Honors in the Major.
Degree Awarded: Summer Semester, 2011.
Date of Defense: April 11, 2011.
Subject(s): Food
Nutrition
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0020
Restrictions on Access: http://creativecommons.org/licenses/by-nc-nd/3.0/
Owner Institution: FSU
Is Part of Series: Honors Theses.

Choose the citation style.
Katz, J. (2011). A Sensitive, Specific, and Robust Enzyme-linked Immunosorbent Assy (elisa) for Macadamia Nut Seed Protein Detection. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0020