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Capillary Electrophoresis Separation of Formaldehyde Cross-Linked Aptamer-Protein Complexes

Title: Capillary Electrophoresis Separation of Formaldehyde Cross-Linked Aptamer-Protein Complexes.
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Name(s): Duren, Joseph, Jr., author
Biochemistry
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2011
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: An Immunoglobulin-E (IgE) DNA aptamer was covalently bound to IgE using a formaldehyde cross-linking method. The resulting complex was then separated from free aptamer by capillary electrophoresis (CE). The bound-to-free peak area ratios were preserved as a result of the cross-linking, which acted as a "freeze-frame" of the reaction in equilibrium. Elevated temperature occurring during CE separations can cause dissociation of non-covalently bound molecules and their targets, decreasing the usefulness of the information obtained. Due to the formaldehyde cross-linking performed, the bound-to-free ratio was preserved over that of the uncross-linked control, which showed a decreased bound-to-free ratio upon heating. Additional trials were run on bovine serum albumin (BSA), anti-BSA, glucagon, and anti-glucagon using the formaldehyde cross-linking method. The results for this section were not conclusive due to difficulties in the separation and mechanistic issues with formaldehyde cross-linking. With the use of formaldehyde cross-linking, complexes that easily dissociate, under stringent separation conditions, can be covalently linked to prevent dissociation and allow for more sensitive analyses to be performed. Keywords:
Identifier: FSU_migr_uhm-0012 (IID)
Keywords: Capillary electrophoresis, Aptamer, Cross-linking
Submitted Note: A Thesis submitted to the Department of Chemistry & Biochemistry in partial fulfillment of the requirements for graduation with Honors in the Major.
Degree Awarded: Spring Semester, 2011.
Date of Defense: April 15, 2011.
Subject(s): Chemistry
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0012
Restrictions on Access: http://creativecommons.org/licenses/by-nc-nd/3.0/
Owner Institution: FSU
Is Part of Series: Honors Theses.

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Duren, J. (2011). Capillary Electrophoresis Separation of Formaldehyde Cross-Linked Aptamer-Protein Complexes. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0012