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E.Coli Growth and Transport in the Presence of Nanosilver under Variable Growth Conditions

Title: E.Coli Growth and Transport in the Presence of Nanosilver under Variable Growth Conditions.
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Name(s): Xie, Weijie, author
Chen, Gang, professor directing thesis
Huang, Wenrui, committee member
Clark, Clayton, committee member
Department of Civil and Environmental Engineering, degree granting department
Florida State University, degree granting institution
Type of Resource: text
Genre: Text
Issuance: monographic
Date Issued: 2014
Publisher: Florida State University
Place of Publication: Tallahassee, Florida
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Nanosilver has the ability to anchor to the bacterial cell wall and subsequently penetrate it, thereby causing structural changes in the cell membrane and death of the cell. The bacterial responses to the presence of nanosilver usually vary depending on the concentration of nanosilver particles, exposure conditions and the bacterial physiological stage. Since bacterial anabolism dependents upon a stoichiometric ratio of carbon and inorganic nutrients, the carbon to nitrogen ratio (C/N) thus plays an important role of bacterial responses to the exposure of nanosilver. This study investigated the responses of Escherichia coli to the exposure of nanosilver under variable growth conditions. It was discovered that E. coli grown under different growth conditions had least resistance to the toxicity of nanosilver when cultured under carbon limited conditions. However, the presence of rhamnolipid, a commonly utilized biosurfactant for soil remediation increased the resistance of E. coli to nanosilver. The transport of E. coli cultured under carbon limited conditions was further studied in silica sand columns. E. coli adsorption in silica sand increased when cultured in the presence of nanosilver. On the contrary, E. coli adsorption in silica sand was significantly reduced when cultured in the presence of rhamnolipid. The results suggest that the resistance of E.coli to nanosilver can be improved by diminishing the carbon limitation in growth condition and the transport impact of nanosilver can be diminished by adding rhamnolipid.
Identifier: FSU_migr_etd-8921 (IID)
Submitted Note: A Thesis submitted to the Department of Civil and Environmental Engineering in partial fulfillment of the requirements for the degree of Master of Science.
Degree Awarded: Spring Semester, 2014.
Date of Defense: April 8, 2014.
Keywords: Echerichia coli, Macro-Nutrient, Nanosilver, Rhamnolipid, Transport
Bibliography Note: Includes bibliographical references.
Advisory Committee: Gang Chen, Professor Directing Thesis; Wenrui Huang, Committee Member; Clayton Clark, Committee Member.
Subject(s): Civil engineering
Environmental engineering
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_etd-8921
Owner Institution: FSU

Choose the citation style.
Xie, W. (2014). E.Coli Growth and Transport in the Presence of Nanosilver under Variable Growth Conditions. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-8921