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Group I and II Metabotropic Glutamate Receptors Are Necessary for the Activity-Dependent Maintenance of Ribosomal Integrity in Chick Auditory Neurons

Title: Group I and II Metabotropic Glutamate Receptors Are Necessary for the Activity-Dependent Maintenance of Ribosomal Integrity in Chick Auditory Neurons.
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Name(s): Nicholas, Alexander H., author
Hyson, Richard L., professor directing thesis
Joiner, Thomas, committee member
Johnson, Frank, committee member
Department of Psychology, degree granting department
Florida State University, degree granting institution
Type of Resource: text
Genre: Text
Issuance: monographic
Date Issued: 2004
Publisher: Florida State University
Place of Publication: Tallahassee, Florida
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Elimination of eighth-nerve activity results in the death of 30% of the neurons in the chick cochlear nucleus, n. magnocellularis (NM). One early event in this cell death cascade is the disruption of ribosomes in NM neurons, which can be observed within one hour following deafferentation. These rapid changes in ribosomal integrity can be visualized using Y10b, a monoclonal antibody that recognizes ribosomal RNA. Previous studies using a brain slice preparation of the avian brain stem auditory system have shown that activation of metabotropic glutamate receptors (mGluRs) is necessary for the activity-dependent maintenance of ribosomal integrity. The purpose of the present study was to determine if group I and/or II mGluRs are necessary for this activity-dependent regulation. This was accomplished by selectively blocking group I or II receptors while unilaterally stimulating the auditory nerve in vitro. Y10b immunostaining served as the marker for ribosomal integrity. In normal media, unilateral stimulation of the auditory nerve resulted in darker Y10b labeling of NM neurons on the stimulated side of the slice. The group I antagonist (RS)-1-aminoindan-1,5-dicarboxylic acid (AIDA) and the group II antagonists LY341495 and (S)- -ethylglutamic acid (EGLU) all prevented the activity-dependent difference in immunolabeling. These data suggest that both group I and II mGluRs play vital roles in the activity-dependent maintenance of ribosomal integrity in NM.
Identifier: FSU_migr_etd-2611 (IID)
Submitted Note: A Thesis submitted to the Department of Psychology in partial fulfillment of the requirements for the degree of Master of Science.
Degree Awarded: Spring Semester, 2004.
Date of Defense: January 23, 2004.
Keywords: Ribosome, Nucleus Magnocellularis, Cell Death, Deafferentation, Avian
Bibliography Note: Includes bibliographical references.
Advisory Committee: Richard L. Hyson, Professor Directing Thesis; Thomas Joiner, Committee Member; Frank Johnson, Committee Member.
Subject(s): Psychology
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_etd-2611
Owner Institution: FSU

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Nicholas, A. H. (2004). Group I and II Metabotropic Glutamate Receptors Are Necessary for the Activity-Dependent Maintenance of Ribosomal Integrity in Chick Auditory Neurons. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-2611