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Increased Activation of ∆Fosb in the Medial Preoptic Area of Male Rats with Sexual Experience

Title: Increased Activation of ∆Fosb in the Medial Preoptic Area of Male Rats with Sexual Experience.
Name(s): Picotte, Katie B., author
Hull, Elaine M., professor directing thesis
Kabbaj, Mohamed, committee member
Licht, Barbara G., committee member
Department of Psychology, degree granting department
Florida State University, degree granting institution
Type of Resource: text
Genre: Text
Issuance: monographic
Date Issued: 2009
Publisher: Florida State University
Place of Publication: Tallahassee, Florida
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The transcription factor ΔFosB is increased in the brain after repeated exposure to several forms of stimuli. The nucleus accumbens (NAc), an area of the brain involved in rewarding behavior, is a site of major ΔFosB activity after exposure to chronic stimuli, including, but not limited to, drugs of abuse, stress, wheel running, sucrose consumption, and sex. The medial preoptic area (MPOA) is an area important for sexual and parental behavior. The immediate-early gene product c-Fos is observed in the medial preoptic nucleus (MPN) after initial sexual experience, and to a greater degree after multiple experiences. c-Fos is also found in the MPOA after parental behavior (i.e. retrieving pups), as is FosB, the full-length transcript of which ΔFosB is a truncated form. ΔFosB has recently been found to be increased in the NAc after multiple sexual experiences; however, FosB and ΔFosB have not been investigated in the MPOA after any amount of sexual experience. For this thesis, we used immunohistochemistry (IHC) to investigate FosB/ΔFosB activation in the MPOA of male rats that were given various amounts of sexual experience, including fifteen, one, and zero sessions. Two groups received fifteen sessions; one group received their last experience one week before sacrifice, whereas the second group received their last experience the day before sacrifice. We found the number of FosB/ΔFosB-immunoreactive cells is increased significantly in the MPN of males given one experience, compared to naïve males and the experienced group receiving their last experience one week before sacrifice; those given long-term sexual experience (up until the day before) were not significantly different from any of the other 3 groups. Western immunblotting was used to determine the molecular identity of the protein being expressed in IHC. It appears that ΔFosB, at 35-37kDa, is expressed in the MPOA after the first sexual experience. Based on these results, it is postulated that ΔFosB in the MPOA may be important for the priming of sensory and/or motor integration in the MPOA with the initial sexual experience of male rats.
Identifier: FSU_migr_etd-0889 (IID)
Submitted Note: A Thesis submitted to the Department of Psychology in partial fulfillment of the requirements for the degree of Master of Science.
Degree Awarded: Degree Awarded: Fall Semester, 2009.
Date of Defense: Date of Defense: July 31, 2009.
Keywords: Male Rats, Sexual Behavior, Medial Preoptic Area, FosB
Bibliography Note: Includes bibliographical references.
Advisory committee: Elaine M. Hull, Professor Directing Thesis; Mohamed Kabbaj, Committee Member; Barbara G. Licht, Committee Member.
Subject(s): Psychology
Persistent Link to This Record:
Owner Institution: FSU

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Picotte, K. B. (2009). Increased Activation of ∆Fosb in the Medial Preoptic Area of Male Rats with Sexual Experience. Retrieved from